In vitro Hemostasis Activity Analysis of Ethanol Extract of Sappan Wood Administration Based on Clotting Time Lee and White Method

  • Ragil Saptaningtyas Bachelor of Applied Sciences of Medical Laboratory Technology, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang
  • Putri Sulfitri Bachelor of applied sciences of Medical Laboratory Technology, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang
  • Annisa Nurul Hikmah Diploma of Medical Laboratory Technology, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang
  • Erma Lestari Diploma of Medical Laboratory Technology, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang
  • Joko Teguh Isworo Bachelor of Nutrition Science, Faculty of Nursing and Health Science, Universitas Muhammadiyah Semarang
  • Mohd Hanif Zulfakar Faculty of Pharmacy of Universiti Kebangsaan Malaysia
Keywords: : Sappan wood, clotting time, Lee and White

Abstract

Hemostasis is a combination of events following vascular injury, including vasoconstriction, platelet aggregation, thrombus formation, recanalization, and debridement. Clotting time is an assessment to determine the time required for blood to clot in vitro. This study aimed to determine the effect of sappan wood ethanol extract on the outcome of the clotting time test using the Lee and White method. This study is experimental, with several samples of 32 divided into three groups, using the Lee and White method clotting time examination. Group C as a control group (1 mL of venous blood only), P1 and P2 as much as 1 mL of venous blood by adding 0.3 grams and 0.4 grams of sappan wood ethanol extract, respectively. The data analysis used was One-way ANOVA with Post hoc LSD test. One-way ANOVA test revealed significant differences between research groups (<0.05). Post hoc LSD results showed a significant difference between P1 and P2 compared to group C. It can be summarized that there is a difference in the results of the Lee and White method clotting time examination between blood with and without sappan wood ethanol extract.

References

1. Lopez, Edgardo Olvera, Brian D. Ballard, A. J. Cardiovascular disease: About Cardiovascular Disease. in Cardiovascular Disease 1–12 (NCBI, 2022).
2. Maharani, A. et al. Cardiovascular Disease Risk Factor Prevalence and Estimated 10-year Cardiovascular Risk Scores in Indonesia: The SMARThealth Extend study. PLoS One 14, 1–13 (2019).
3. Berger, J. S. Aspirin for Primary Prevention - Time to Rethink Our Approach. JAMA Netw. Open 5, E2210144 (2022).
4. Lapelusa, A., Dave, H. D. & Federation, R. Physiology , Hemostasis. 1–9 (2023).
5. Zaidi, A. & Green, L. Review Article: Physiology of Haemostasis. Anaesthesis Intensive Care Med. 20, 152–158 (2019).
6. Sari dan Suhartati, R., Sari, R., Suhartati Balai Litbang Lingkungan Hidup dan Kehutanan Makassar Jl Perintis Kemerdekaan Km, dan, Selatan, S. & pos, K. Secang (Caesalpinia sappan L.) : Tumbuhan Herbal Kaya Antioksidan. 57–68 (2010).
7. Nomer, N. M. G. R., Duniaji, A. S. & Nocianitri, K. A. Kandungan Senyawa Flavonoid dan Antosianin Ekstrak Kayu Secang (Caesalpinia sappan L.) serta Aktivitas Antibakteri Terhadap Vibrio cholerae. J. Ilmu dan Teknol. Pangan 8, 216 (2019).
8. Chang, Y. et al. Brazilin Isolated from Caesalpinia sappan L. Acts As a Novel Collagen Receptor Agonist in Human Platelets. J. Biomed. Sci. 20, 4 (2013).
9. Ciesla, B. Basic Hematologica Principle. Hematology in Practice (2007).
10. Alkarithi, G., Duval, C., Shi, Y., Macrae, F. L. & Ariëns, R. A. S. Thrombus Structural Composition in Cardiovascular Disease. Arterioscler. Thromb. Vasc. Biol. 41, 2370–2383 (2021).
11. Little, P. J., Askew, C. D., Xu, S. & Kamato, D. Endothelial Dysfunction and Cardiovascular Disease: History and Analysis of The Clinical Utility of The Relationship. Biomedicines 9, 1–9 (2021).
12. Widowati, W. P. I. K. Uji Fitokimia dan Potensi Antioksidan Ekstrak Etanol Kayu Secang ( Caesalpinia sappan L .). J. Kedokt. Maranatha 11, 23–31 (2011).
13. Mu’nisa, A., Hala, Y. & Muflihunna, A. Analysis of Phenols and Antioxidants Infused Sappan. Int. J. Sci. Dev. Res. 2, 89–93 (2017).
Published
2024-10-25
How to Cite
1.
Saptaningtyas R, Putri Sulfitri, Annisa Nurul Hikmah, Erma Lestari, Joko Teguh Isworo, Mohd Hanif Zulfakar. In vitro Hemostasis Activity Analysis of Ethanol Extract of Sappan Wood Administration Based on Clotting Time Lee and White Method. woh [Internet]. 2024Oct.25 [cited 2024Nov.23];7(4):432 -437. Available from: http://103.133.36.92/index.php/woh/article/view/1423
Section
Articles